![]() For this reason, every antibody comes with in-depth information including details of the immunogen used in its generation, and recommended conditions for suggested applications like western blotting. ![]() We understand the importance of transparency in our production and testing processes, guiding customers to the correct reagents for their research needs. Using Bio-Rad’s stain-free imaging technology, we monitor protein loading consistency and transfer efficiency. Hoechst was used as nuclear stain (blue) and imaged with a 20x objective. B, HeLa cells stained with 5 µg/ml Mouse Anti-GPI Antibody ( VMA00348, green). A, western blot analysis on multiple cell lines probed with PCNA Antibody ( VMA00018 ) arrow points to PCNA (molecular weight 29 kDa). Western blots are carried out by scientists who scrutinize the data comparing relevant bioinformatic database references against signal-to-background ratio, ensuring that only the best antibodies are selected as PrecisionAb Antibodies.įig. For each antibody, we carefully review the literature to identify lysates expected to express the target and use this to evaluate the antibody. Where relevant, negative controls and other important samples, such as human serum and human or rodent tissue lysates, are included. PrecisionAb Antibodies are evaluated against multiple biologically relevant cell lysates and tissues that express the endogenous target (Figure 1). The PrecisionAb Antibody range, rigorously tested in western blotting for specificity and reproducibility, is designed to help researchers produce the highest-quality western blotting data. We are also expanding the applications PrecisionAb Antibodies are tested in to include immunofluorescence (IF) and immunoprecipitation (IP) (Figure 1). These include genetic strategies, orthogonal strategies, independent antibody strategies, expression of tagged proteins, and immunocapture followed by mass spectrometry (MS).Īt Bio-Rad, we are committed to providing our customers with reliable research tools and are constantly raising our standards for antibody quality. The IWGAV defined five validation pillars that provide a framework for validation testing. To address this, the International Working Group on Antibody Validation (IWGAV) has suggested broad guidelines for antibody validation (Uhlen et al. “Antibody validation is the experimental proof and documentation that a specific antibody is suitable for an intended application or purpose” (Weller 2018). In recent years, a number of studies have questioned the reliability of published data, and highlighted how poor-quality antibodies have contributed to a lack of consistent results (Bordeaux et al. Antibodies are one of the most important research tools for biomedical science.
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